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    Merck & Co edta free protease inhibitor cocktail merck
    Edta Free Protease Inhibitor Cocktail Merck, supplied by Merck & Co, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/edta+free+protease+inhibitor+cocktail+merck/cocktail+inhibitor+protease/pm40992376-772-182-186
    Average 86 stars, based on 1 article reviews
    edta free protease inhibitor cocktail merck - by Bioz Stars, 2026-09
    86/100 stars

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    Article Title: Dynamic recruitment of Munc13 primes docked secretory granules for exocytosis.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Monoclonal Anti-Syntaxin antibody, Mouse Sigma Aldrich Cat# S0664; RRID: AB_477483 Munc18-1 antibody Synaptic Systems Cat# 116 002; RRID: AB_887736 Munc13-1 antibody Synaptic Systems Cat# 126 102; RRID: AB_887734 Munc13-1 antibody, affinity purified Synaptic Systems Cat# 126 103; RRID: AB_887733 Munc13-2 antibody Synaptic Systems Cat# 126 203; RRID: AB_2619807 anti Mouse IgG (H + L) Alexa 568 Invitrogen Cat# A-11004; RRID: AB_2534072 Immuno-Star Goat Anti-Rabbit (GAR)-HRP Conjugate Bio-Rad Cat# 1705046 Bacterial and virus strains RIP2 adenovirus Virus Shuai et al. 67 N/A Biological samples Human islet donor, T2D Nordic Network for Clinical Islet Transplantation H2686 Human islet donor, T2D Nordic Network for Clinical Islet Transplantation H2689 Human islet donor, ND Nordic Network for Clinical Islet Transplantation R538 Human islet donor, ND Nordic Network for Clinical Islet Transplantation R2681 Human islet donor, ND Nordic Network for Clinical Islet Transplantation R2691 Chemicals, peptides, and recombinant proteins Phorbol 12-Myristate 13-Acetate Sigma-Aldrich Cat# P1585-1MG BAPTA Merck Millipore Cat# 196418-1GM EGTA Sigma-Aldrich Cat# E4378-25G M-PER, Mammalian Protein Extraction Reagent Thermo Scientific Cat# 78501 cOmplete, Mini, EDTA-free Protease Inhibitor Cocktail Merck Cat# 11836170001 Protein G plus/Protein A Agarose Suspension Merck Cat# IP05 Lipofectamin 2000 Invitrogen Cat# 11668-019 Lipofectamin 3000 Invitrogen Cat# L3000001 Puromycin Dihydrochloride Thermo Fisher Cat# A1113803 Critical commercial assays Neon TM NxT Buffer kit 10 mL Invitrogen Cat# N1096B Neon TM NxT Disposable 96 tips & tubes 10 mL Invitrogen Cat# N1096K Neon TM NxT Buffer kit 100 mL Invitrogen Cat# N10096B Neon TM NxT Disposable 96 tips & tubes 100 mL Invitrogen Cat# N10096K Trans-Blot Turbo Transfer System, PVDF membrane Bio-Rad Cat# 1704156 4-20% Mini-PROTEAN TXG Precast Protein Gel Bio-Rad Cat# 4561094 Clarity Max Western ECL Substrate Bio-Rad Cat# 1705060 EveryBlot Blocking Buffer Bio-Rad Cat# 12010020 Phire Tissue Direct PCR Master Mix Thermo Scientific Cat# F170S Deposited data Humanislet.com, Entrez:23025-unc-13 homolog A Ewald et al. 34 N/A Codes generated for analysis This study Zendo:https://doi.org/10.5281/ zendo.16689607 Rat Munc13.1-EGFP GenBank GeneBank: PP663251 (Continued on next page) Cell Reports 44, 116301, October 28, 2025 15

    Produced:

    Article Title: Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-V5(Pk) Bio-Rad Cat# MCA1360; RRID:AB_322378 Monoclonal ANTI-FLAG® M2 antibody produced in mouse Sigma-Aldrich Cat# F3165; RRID:AB_259529 Goat anti-myc tag antibody, affinity purified Bethyl Laboratories Cat# A190-104A; RRID:AB_66864 Mouse monoclonal anti-acetylated Smc3 Gift from the Shirahige laboratory N/A Chemicals, peptides, and recombinant proteins Rabbit IgG-Agarose Merck Cat# A2909 TALON Metal Affinity Resin Clonetech Cat# 635502 HiTrap Heparin HP 1ml Cytiva Cat# 17-0407-01 HiTrap SP HP 1ml Cytiva Cat# 29-0513-24 Superose 6 Increase 10/300 GL Cytiva Cat# 29-0915-96 Superdex 200 Increase 10/300 GL Cytiva Cat# 28-9909-44 Superdex 75 10/300 GL Cytiva Cat# 17-5174-01 cOmplete, EDTA-free Protease Inhibitor Cocktail Merck Cat# 04693132001 Pefabloc SC Roche Cat# 11429876001 SYBR Gold nucleic acid gel stain ThermoFisher Cat# S11494 Acetyl coenzyme A lithium salt Merck Cat# A2181 T5 Exonuclease NEB Cat # M0663 ATP ThermoFisher Cat# R0441 TCEP Fluorochem Limited Cat# M02624 Proteinase K Merck Cat# 107393 Calmodulin Affinity Resin Agilent Cat# 214303 ANTI-FLAG® M2 Affinity Gel Merck Cat# A2220 SIGMAFASTTM Protease Inhibitor Cocktail Tablets, EDTA-Free Sigma-Aldrich Cat# S8830 QIAGEN Plasmid Mega Kit QIAGEN Cat# 12181 5 mL, Open-Top Thinwall Ultra-Clear Tube, BECKMAN COUTER Cat# 344057 [α- 32 P]dCTP, 3000 Ci/mmol, 20 mCi/ml HARTMANN ANALYTIC Cat# FP-205H Amersham MicroSpin G-50 Columns Cytiva Cat# 27533001 BMOE (bis-maleimidoethane) Thermo Fisher SCIENTIFC Cat# 22323 Dpn I NEB Cat# R0176 Nb.BssSI NEB Cat# R0681 DynabeadsTM Protein G for Immunoprecipitation Invitrogen Cat# 10004D Experimental models: Yeast strains Most yeast strains were previously described. ..

    Affinity Purification:

    Article Title: Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-V5(Pk) Bio-Rad Cat# MCA1360; RRID:AB_322378 Monoclonal ANTI-FLAG® M2 antibody produced in mouse Sigma-Aldrich Cat# F3165; RRID:AB_259529 Goat anti-myc tag antibody, affinity purified Bethyl Laboratories Cat# A190-104A; RRID:AB_66864 Mouse monoclonal anti-acetylated Smc3 Gift from the Shirahige laboratory N/A Chemicals, peptides, and recombinant proteins Rabbit IgG-Agarose Merck Cat# A2909 TALON Metal Affinity Resin Clonetech Cat# 635502 HiTrap Heparin HP 1ml Cytiva Cat# 17-0407-01 HiTrap SP HP 1ml Cytiva Cat# 29-0513-24 Superose 6 Increase 10/300 GL Cytiva Cat# 29-0915-96 Superdex 200 Increase 10/300 GL Cytiva Cat# 28-9909-44 Superdex 75 10/300 GL Cytiva Cat# 17-5174-01 cOmplete, EDTA-free Protease Inhibitor Cocktail Merck Cat# 04693132001 Pefabloc SC Roche Cat# 11429876001 SYBR Gold nucleic acid gel stain ThermoFisher Cat# S11494 Acetyl coenzyme A lithium salt Merck Cat# A2181 T5 Exonuclease NEB Cat # M0663 ATP ThermoFisher Cat# R0441 TCEP Fluorochem Limited Cat# M02624 Proteinase K Merck Cat# 107393 Calmodulin Affinity Resin Agilent Cat# 214303 ANTI-FLAG® M2 Affinity Gel Merck Cat# A2220 SIGMAFASTTM Protease Inhibitor Cocktail Tablets, EDTA-Free Sigma-Aldrich Cat# S8830 QIAGEN Plasmid Mega Kit QIAGEN Cat# 12181 5 mL, Open-Top Thinwall Ultra-Clear Tube, BECKMAN COUTER Cat# 344057 [α- 32 P]dCTP, 3000 Ci/mmol, 20 mCi/ml HARTMANN ANALYTIC Cat# FP-205H Amersham MicroSpin G-50 Columns Cytiva Cat# 27533001 BMOE (bis-maleimidoethane) Thermo Fisher SCIENTIFC Cat# 22323 Dpn I NEB Cat# R0176 Nb.BssSI NEB Cat# R0681 DynabeadsTM Protein G for Immunoprecipitation Invitrogen Cat# 10004D Experimental models: Yeast strains Most yeast strains were previously described. ..

    Recombinant:

    Article Title: Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-V5(Pk) Bio-Rad Cat# MCA1360; RRID:AB_322378 Monoclonal ANTI-FLAG® M2 antibody produced in mouse Sigma-Aldrich Cat# F3165; RRID:AB_259529 Goat anti-myc tag antibody, affinity purified Bethyl Laboratories Cat# A190-104A; RRID:AB_66864 Mouse monoclonal anti-acetylated Smc3 Gift from the Shirahige laboratory N/A Chemicals, peptides, and recombinant proteins Rabbit IgG-Agarose Merck Cat# A2909 TALON Metal Affinity Resin Clonetech Cat# 635502 HiTrap Heparin HP 1ml Cytiva Cat# 17-0407-01 HiTrap SP HP 1ml Cytiva Cat# 29-0513-24 Superose 6 Increase 10/300 GL Cytiva Cat# 29-0915-96 Superdex 200 Increase 10/300 GL Cytiva Cat# 28-9909-44 Superdex 75 10/300 GL Cytiva Cat# 17-5174-01 cOmplete, EDTA-free Protease Inhibitor Cocktail Merck Cat# 04693132001 Pefabloc SC Roche Cat# 11429876001 SYBR Gold nucleic acid gel stain ThermoFisher Cat# S11494 Acetyl coenzyme A lithium salt Merck Cat# A2181 T5 Exonuclease NEB Cat # M0663 ATP ThermoFisher Cat# R0441 TCEP Fluorochem Limited Cat# M02624 Proteinase K Merck Cat# 107393 Calmodulin Affinity Resin Agilent Cat# 214303 ANTI-FLAG® M2 Affinity Gel Merck Cat# A2220 SIGMAFASTTM Protease Inhibitor Cocktail Tablets, EDTA-Free Sigma-Aldrich Cat# S8830 QIAGEN Plasmid Mega Kit QIAGEN Cat# 12181 5 mL, Open-Top Thinwall Ultra-Clear Tube, BECKMAN COUTER Cat# 344057 [α- 32 P]dCTP, 3000 Ci/mmol, 20 mCi/ml HARTMANN ANALYTIC Cat# FP-205H Amersham MicroSpin G-50 Columns Cytiva Cat# 27533001 BMOE (bis-maleimidoethane) Thermo Fisher SCIENTIFC Cat# 22323 Dpn I NEB Cat# R0176 Nb.BssSI NEB Cat# R0681 DynabeadsTM Protein G for Immunoprecipitation Invitrogen Cat# 10004D Experimental models: Yeast strains Most yeast strains were previously described. ..

    Article Title: Ubiquitin precursor with C-terminal extension promotes proteostasis and longevity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-UbN77 Pineda N/A Rabbit polyclonal anti-ATG32 Pineda N/A Rabbit polyclonal anti-Fzo1 Simoes et al., 2018 69 N/A Rabbit polyclonal anti-MBP-Pho8 (mtALP) Mendl et al., 2011 26 N/A Mouse monoclonal anti-GFP Roche #11814460001; RRID:AB_390913 anti-Bmh1/2 W. Neupert N/A Mouse monoclonal anti-Pgk1 Abcam #ab197960; RRID:AB_2756444 Mouse monoclonal anti-Ubiquitin (P4D1) Cell Signaling #3936; RRID: AB_331292 Rat monoclonal anti-HA Roche #11867423001; RRID: AB_390918 Rabbit polyclonal anti-Flag Merck F7425; RRID: AB_439687 Rabbit polyclonal anti-Myc Merck (Sigma) C3956; RRID: AB_439680 Mouse monoclonal anti-βGalactosidase Promega #Z3781; RRID:AB_430877 Mouse monoclonal anti-Atg11 C. Kraft n/a Anti-Ape1 C. Kraft n/a Rabbit monoclonal anti-Ubc6 T. Sommer n/a Rabbit monoclonal anti-Atg8 K. Okamoto n/a Bacterial and virus strains DH5α competent cells ThermoFisher Scientific # 18265017 Rosetta (DE3) pLysS competent cells Merck #71137 Chemicals, peptides, and recombinant proteins Ponceau S Serva #33429.02 Cabonyl cyanide 3-chlorophenylhydrazon (CCCP) Merck #C2759 Zinc chloride Carl Roth #3533.2 L-Canavanine Merck #C1625 BioTracker 640 Red C2(FM4-64) Merck #SCT127 MG132 InSolution 10mM in DMSO Calbiochem #474791 Cycloheximide In Solution 100mg/ml in DMSO Merck #C4859 Copper (II) Sulphate pentahydrate Merck #209198 Rapamycin Merck #553210 cOmplete, EDTA-free protease inhibitor cocktail Merck #11873580001 p-nitrophenylphosphate Merck #487663 DTT MP #100597 Color Prestained Protein Standard, Broad Range NEB #P7719 Lauryl maltose neopentyl glycol (NG310) Biozol #ANA-NG310 EZview Red Anti-FLAG M2 Affinity Gel Merck #F2426 EZview Red Anti-HA Affinity Gel Merck #E6779 ESTLHLVLR*LR*GGN JPT Peptide Technologies N/A Critical commercial assays GE Healthcare AmershamTM ECL TM Prime Amersham #RPN2232 Deposited data Mendeley data This study https://doi.org/10.17632/mgsw2pcnhc.1 PRIDE (proteomics) This study PRIDE: PXD065783 (Continued on next page) e1 Molecular Cell 85, 3677–3693.e1–e7, October 2, 2025 ..

    Protease Inhibitor:

    Article Title: Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-V5(Pk) Bio-Rad Cat# MCA1360; RRID:AB_322378 Monoclonal ANTI-FLAG® M2 antibody produced in mouse Sigma-Aldrich Cat# F3165; RRID:AB_259529 Goat anti-myc tag antibody, affinity purified Bethyl Laboratories Cat# A190-104A; RRID:AB_66864 Mouse monoclonal anti-acetylated Smc3 Gift from the Shirahige laboratory N/A Chemicals, peptides, and recombinant proteins Rabbit IgG-Agarose Merck Cat# A2909 TALON Metal Affinity Resin Clonetech Cat# 635502 HiTrap Heparin HP 1ml Cytiva Cat# 17-0407-01 HiTrap SP HP 1ml Cytiva Cat# 29-0513-24 Superose 6 Increase 10/300 GL Cytiva Cat# 29-0915-96 Superdex 200 Increase 10/300 GL Cytiva Cat# 28-9909-44 Superdex 75 10/300 GL Cytiva Cat# 17-5174-01 cOmplete, EDTA-free Protease Inhibitor Cocktail Merck Cat# 04693132001 Pefabloc SC Roche Cat# 11429876001 SYBR Gold nucleic acid gel stain ThermoFisher Cat# S11494 Acetyl coenzyme A lithium salt Merck Cat# A2181 T5 Exonuclease NEB Cat # M0663 ATP ThermoFisher Cat# R0441 TCEP Fluorochem Limited Cat# M02624 Proteinase K Merck Cat# 107393 Calmodulin Affinity Resin Agilent Cat# 214303 ANTI-FLAG® M2 Affinity Gel Merck Cat# A2220 SIGMAFASTTM Protease Inhibitor Cocktail Tablets, EDTA-Free Sigma-Aldrich Cat# S8830 QIAGEN Plasmid Mega Kit QIAGEN Cat# 12181 5 mL, Open-Top Thinwall Ultra-Clear Tube, BECKMAN COUTER Cat# 344057 [α- 32 P]dCTP, 3000 Ci/mmol, 20 mCi/ml HARTMANN ANALYTIC Cat# FP-205H Amersham MicroSpin G-50 Columns Cytiva Cat# 27533001 BMOE (bis-maleimidoethane) Thermo Fisher SCIENTIFC Cat# 22323 Dpn I NEB Cat# R0176 Nb.BssSI NEB Cat# R0681 DynabeadsTM Protein G for Immunoprecipitation Invitrogen Cat# 10004D Experimental models: Yeast strains Most yeast strains were previously described. ..

    Article Title: Ubiquitin precursor with C-terminal extension promotes proteostasis and longevity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-UbN77 Pineda N/A Rabbit polyclonal anti-ATG32 Pineda N/A Rabbit polyclonal anti-Fzo1 Simoes et al., 2018 69 N/A Rabbit polyclonal anti-MBP-Pho8 (mtALP) Mendl et al., 2011 26 N/A Mouse monoclonal anti-GFP Roche #11814460001; RRID:AB_390913 anti-Bmh1/2 W. Neupert N/A Mouse monoclonal anti-Pgk1 Abcam #ab197960; RRID:AB_2756444 Mouse monoclonal anti-Ubiquitin (P4D1) Cell Signaling #3936; RRID: AB_331292 Rat monoclonal anti-HA Roche #11867423001; RRID: AB_390918 Rabbit polyclonal anti-Flag Merck F7425; RRID: AB_439687 Rabbit polyclonal anti-Myc Merck (Sigma) C3956; RRID: AB_439680 Mouse monoclonal anti-βGalactosidase Promega #Z3781; RRID:AB_430877 Mouse monoclonal anti-Atg11 C. Kraft n/a Anti-Ape1 C. Kraft n/a Rabbit monoclonal anti-Ubc6 T. Sommer n/a Rabbit monoclonal anti-Atg8 K. Okamoto n/a Bacterial and virus strains DH5α competent cells ThermoFisher Scientific # 18265017 Rosetta (DE3) pLysS competent cells Merck #71137 Chemicals, peptides, and recombinant proteins Ponceau S Serva #33429.02 Cabonyl cyanide 3-chlorophenylhydrazon (CCCP) Merck #C2759 Zinc chloride Carl Roth #3533.2 L-Canavanine Merck #C1625 BioTracker 640 Red C2(FM4-64) Merck #SCT127 MG132 InSolution 10mM in DMSO Calbiochem #474791 Cycloheximide In Solution 100mg/ml in DMSO Merck #C4859 Copper (II) Sulphate pentahydrate Merck #209198 Rapamycin Merck #553210 cOmplete, EDTA-free protease inhibitor cocktail Merck #11873580001 p-nitrophenylphosphate Merck #487663 DTT MP #100597 Color Prestained Protein Standard, Broad Range NEB #P7719 Lauryl maltose neopentyl glycol (NG310) Biozol #ANA-NG310 EZview Red Anti-FLAG M2 Affinity Gel Merck #F2426 EZview Red Anti-HA Affinity Gel Merck #E6779 ESTLHLVLR*LR*GGN JPT Peptide Technologies N/A Critical commercial assays GE Healthcare AmershamTM ECL TM Prime Amersham #RPN2232 Deposited data Mendeley data This study https://doi.org/10.17632/mgsw2pcnhc.1 PRIDE (proteomics) This study PRIDE: PXD065783 (Continued on next page) e1 Molecular Cell 85, 3677–3693.e1–e7, October 2, 2025 ..

    Staining:

    Article Title: Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-V5(Pk) Bio-Rad Cat# MCA1360; RRID:AB_322378 Monoclonal ANTI-FLAG® M2 antibody produced in mouse Sigma-Aldrich Cat# F3165; RRID:AB_259529 Goat anti-myc tag antibody, affinity purified Bethyl Laboratories Cat# A190-104A; RRID:AB_66864 Mouse monoclonal anti-acetylated Smc3 Gift from the Shirahige laboratory N/A Chemicals, peptides, and recombinant proteins Rabbit IgG-Agarose Merck Cat# A2909 TALON Metal Affinity Resin Clonetech Cat# 635502 HiTrap Heparin HP 1ml Cytiva Cat# 17-0407-01 HiTrap SP HP 1ml Cytiva Cat# 29-0513-24 Superose 6 Increase 10/300 GL Cytiva Cat# 29-0915-96 Superdex 200 Increase 10/300 GL Cytiva Cat# 28-9909-44 Superdex 75 10/300 GL Cytiva Cat# 17-5174-01 cOmplete, EDTA-free Protease Inhibitor Cocktail Merck Cat# 04693132001 Pefabloc SC Roche Cat# 11429876001 SYBR Gold nucleic acid gel stain ThermoFisher Cat# S11494 Acetyl coenzyme A lithium salt Merck Cat# A2181 T5 Exonuclease NEB Cat # M0663 ATP ThermoFisher Cat# R0441 TCEP Fluorochem Limited Cat# M02624 Proteinase K Merck Cat# 107393 Calmodulin Affinity Resin Agilent Cat# 214303 ANTI-FLAG® M2 Affinity Gel Merck Cat# A2220 SIGMAFASTTM Protease Inhibitor Cocktail Tablets, EDTA-Free Sigma-Aldrich Cat# S8830 QIAGEN Plasmid Mega Kit QIAGEN Cat# 12181 5 mL, Open-Top Thinwall Ultra-Clear Tube, BECKMAN COUTER Cat# 344057 [α- 32 P]dCTP, 3000 Ci/mmol, 20 mCi/ml HARTMANN ANALYTIC Cat# FP-205H Amersham MicroSpin G-50 Columns Cytiva Cat# 27533001 BMOE (bis-maleimidoethane) Thermo Fisher SCIENTIFC Cat# 22323 Dpn I NEB Cat# R0176 Nb.BssSI NEB Cat# R0681 DynabeadsTM Protein G for Immunoprecipitation Invitrogen Cat# 10004D Experimental models: Yeast strains Most yeast strains were previously described. ..

    Plasmid Preparation:

    Article Title: Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-V5(Pk) Bio-Rad Cat# MCA1360; RRID:AB_322378 Monoclonal ANTI-FLAG® M2 antibody produced in mouse Sigma-Aldrich Cat# F3165; RRID:AB_259529 Goat anti-myc tag antibody, affinity purified Bethyl Laboratories Cat# A190-104A; RRID:AB_66864 Mouse monoclonal anti-acetylated Smc3 Gift from the Shirahige laboratory N/A Chemicals, peptides, and recombinant proteins Rabbit IgG-Agarose Merck Cat# A2909 TALON Metal Affinity Resin Clonetech Cat# 635502 HiTrap Heparin HP 1ml Cytiva Cat# 17-0407-01 HiTrap SP HP 1ml Cytiva Cat# 29-0513-24 Superose 6 Increase 10/300 GL Cytiva Cat# 29-0915-96 Superdex 200 Increase 10/300 GL Cytiva Cat# 28-9909-44 Superdex 75 10/300 GL Cytiva Cat# 17-5174-01 cOmplete, EDTA-free Protease Inhibitor Cocktail Merck Cat# 04693132001 Pefabloc SC Roche Cat# 11429876001 SYBR Gold nucleic acid gel stain ThermoFisher Cat# S11494 Acetyl coenzyme A lithium salt Merck Cat# A2181 T5 Exonuclease NEB Cat # M0663 ATP ThermoFisher Cat# R0441 TCEP Fluorochem Limited Cat# M02624 Proteinase K Merck Cat# 107393 Calmodulin Affinity Resin Agilent Cat# 214303 ANTI-FLAG® M2 Affinity Gel Merck Cat# A2220 SIGMAFASTTM Protease Inhibitor Cocktail Tablets, EDTA-Free Sigma-Aldrich Cat# S8830 QIAGEN Plasmid Mega Kit QIAGEN Cat# 12181 5 mL, Open-Top Thinwall Ultra-Clear Tube, BECKMAN COUTER Cat# 344057 [α- 32 P]dCTP, 3000 Ci/mmol, 20 mCi/ml HARTMANN ANALYTIC Cat# FP-205H Amersham MicroSpin G-50 Columns Cytiva Cat# 27533001 BMOE (bis-maleimidoethane) Thermo Fisher SCIENTIFC Cat# 22323 Dpn I NEB Cat# R0176 Nb.BssSI NEB Cat# R0681 DynabeadsTM Protein G for Immunoprecipitation Invitrogen Cat# 10004D Experimental models: Yeast strains Most yeast strains were previously described. ..

    Immunoprecipitation:

    Article Title: Biochemical reconstitution of sister chromatid cohesion establishment during DNA replication.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse monoclonal anti-V5(Pk) Bio-Rad Cat# MCA1360; RRID:AB_322378 Monoclonal ANTI-FLAG® M2 antibody produced in mouse Sigma-Aldrich Cat# F3165; RRID:AB_259529 Goat anti-myc tag antibody, affinity purified Bethyl Laboratories Cat# A190-104A; RRID:AB_66864 Mouse monoclonal anti-acetylated Smc3 Gift from the Shirahige laboratory N/A Chemicals, peptides, and recombinant proteins Rabbit IgG-Agarose Merck Cat# A2909 TALON Metal Affinity Resin Clonetech Cat# 635502 HiTrap Heparin HP 1ml Cytiva Cat# 17-0407-01 HiTrap SP HP 1ml Cytiva Cat# 29-0513-24 Superose 6 Increase 10/300 GL Cytiva Cat# 29-0915-96 Superdex 200 Increase 10/300 GL Cytiva Cat# 28-9909-44 Superdex 75 10/300 GL Cytiva Cat# 17-5174-01 cOmplete, EDTA-free Protease Inhibitor Cocktail Merck Cat# 04693132001 Pefabloc SC Roche Cat# 11429876001 SYBR Gold nucleic acid gel stain ThermoFisher Cat# S11494 Acetyl coenzyme A lithium salt Merck Cat# A2181 T5 Exonuclease NEB Cat # M0663 ATP ThermoFisher Cat# R0441 TCEP Fluorochem Limited Cat# M02624 Proteinase K Merck Cat# 107393 Calmodulin Affinity Resin Agilent Cat# 214303 ANTI-FLAG® M2 Affinity Gel Merck Cat# A2220 SIGMAFASTTM Protease Inhibitor Cocktail Tablets, EDTA-Free Sigma-Aldrich Cat# S8830 QIAGEN Plasmid Mega Kit QIAGEN Cat# 12181 5 mL, Open-Top Thinwall Ultra-Clear Tube, BECKMAN COUTER Cat# 344057 [α- 32 P]dCTP, 3000 Ci/mmol, 20 mCi/ml HARTMANN ANALYTIC Cat# FP-205H Amersham MicroSpin G-50 Columns Cytiva Cat# 27533001 BMOE (bis-maleimidoethane) Thermo Fisher SCIENTIFC Cat# 22323 Dpn I NEB Cat# R0176 Nb.BssSI NEB Cat# R0681 DynabeadsTM Protein G for Immunoprecipitation Invitrogen Cat# 10004D Experimental models: Yeast strains Most yeast strains were previously described. ..

    Virus:

    Article Title: Ubiquitin precursor with C-terminal extension promotes proteostasis and longevity.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal anti-UbN77 Pineda N/A Rabbit polyclonal anti-ATG32 Pineda N/A Rabbit polyclonal anti-Fzo1 Simoes et al., 2018 69 N/A Rabbit polyclonal anti-MBP-Pho8 (mtALP) Mendl et al., 2011 26 N/A Mouse monoclonal anti-GFP Roche #11814460001; RRID:AB_390913 anti-Bmh1/2 W. Neupert N/A Mouse monoclonal anti-Pgk1 Abcam #ab197960; RRID:AB_2756444 Mouse monoclonal anti-Ubiquitin (P4D1) Cell Signaling #3936; RRID: AB_331292 Rat monoclonal anti-HA Roche #11867423001; RRID: AB_390918 Rabbit polyclonal anti-Flag Merck F7425; RRID: AB_439687 Rabbit polyclonal anti-Myc Merck (Sigma) C3956; RRID: AB_439680 Mouse monoclonal anti-βGalactosidase Promega #Z3781; RRID:AB_430877 Mouse monoclonal anti-Atg11 C. Kraft n/a Anti-Ape1 C. Kraft n/a Rabbit monoclonal anti-Ubc6 T. Sommer n/a Rabbit monoclonal anti-Atg8 K. Okamoto n/a Bacterial and virus strains DH5α competent cells ThermoFisher Scientific # 18265017 Rosetta (DE3) pLysS competent cells Merck #71137 Chemicals, peptides, and recombinant proteins Ponceau S Serva #33429.02 Cabonyl cyanide 3-chlorophenylhydrazon (CCCP) Merck #C2759 Zinc chloride Carl Roth #3533.2 L-Canavanine Merck #C1625 BioTracker 640 Red C2(FM4-64) Merck #SCT127 MG132 InSolution 10mM in DMSO Calbiochem #474791 Cycloheximide In Solution 100mg/ml in DMSO Merck #C4859 Copper (II) Sulphate pentahydrate Merck #209198 Rapamycin Merck #553210 cOmplete, EDTA-free protease inhibitor cocktail Merck #11873580001 p-nitrophenylphosphate Merck #487663 DTT MP #100597 Color Prestained Protein Standard, Broad Range NEB #P7719 Lauryl maltose neopentyl glycol (NG310) Biozol #ANA-NG310 EZview Red Anti-FLAG M2 Affinity Gel Merck #F2426 EZview Red Anti-HA Affinity Gel Merck #E6779 ESTLHLVLR*LR*GGN JPT Peptide Technologies N/A Critical commercial assays GE Healthcare AmershamTM ECL TM Prime Amersham #RPN2232 Deposited data Mendeley data This study https://doi.org/10.17632/mgsw2pcnhc.1 PRIDE (proteomics) This study PRIDE: PXD065783 (Continued on next page) e1 Molecular Cell 85, 3677–3693.e1–e7, October 2, 2025 ..



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